Transcriptome Resources

Transcriptomes of vector arthropods and non-vector, medically important arthropods and snakes

Overview

Salivary transcriptomes (sialome) of ticks
Salivary transcriptomes (sialome) of blood feeding insects
Non-blood feeding sialotranscriptomes
Whole Organism Transcriptome
Poisonous animals
ReAnoXcel: an Anopheles gambiae protein database [Maintained]
AnoXcel: an Anopheles gambiae protein database [Old Dataset - Dec 2004]

Salivary transcriptomes (sialome) of ticks:

Argas monolakensis (Mans BJ, Andersen JF, Schwan TG, Ribeiro JM. (2008) Characterization of anti-hemostatic factors in the argasid, Argas monolakensis: Implications for the evolution of blood-feeding in the soft tick family. Insect Biochem Mol Biol. 38:22-41).

• PMID: 18070663
• Supplemental Table 1
• Supplemental Table 2 [Standalone]

Ornithodoros coriaceus (Francischetti IM, Meng Z, Mans BJ, Gudderra N, Hall M, Veenstra TD, Pham VM, Kotsyfakis M, Ribeiro JM. (2008) An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus. J Proteomics. 71(5):493-512).

• PMID: 18725333
• Supplemental Table S1
• Supplemental Table S2 [Standalone]
  

Ornithodoros parkeri (Francischetti I.M., Mans B.J., Meng Z., Gudderra N., Veenstra T.D., Pham V.M., Ribeiro J.M. (2008) An insight into the sialome of the soft tick, Ornithodorus parkeri. Insect Biochem Mol Biol. 38:1-21).

• PMID: 18070662
• Supplemental Table 1
• Supplemental Table 2 [Standalone]
  

Ixodes scapularis (Valenzuela, J. G., Francischetti, I. M. B., Pham, V. M., Garfield, M. K., Mather, T. N. and Ribeiro, J. M. C. (2002). Exploring the sialome of the tick, Ixodes scapularis. J. Exp. Biol. 205, 2843-64).

• PMID: 12177149
• Ix_scapularis_sialome.pdf
• Ix_scapularis_sialome_TableI.ZIP [Correspond to publication data, not updated, Standalone]
• Ix_scapularis_sialome_TableII.ZIP [Correspond to publication data, not updated, Standalone]
• Updated Ixodes scapularis sialome (José M. C. Ribeiro , Francisco Alarcon-Chaidez, Ivo M. B. Francischetti , Ben J. Mans, Thomas N. Mather , Jesus G. Valenzuela, and Stephen K. Wikel. (2006) An annotated catalog of salivary gland transcripts from Ixodes scapularis ticks. Insect Biochem Mol Biol.  36(2):111-29
  • Manuscript
  • Tables
  • Supplemental Table 1
  • Supplemental Table 2

Ixodes pacificus (Francischetti, I.M., Pham, V.M., Mans, B., Andersen, J.F., Mather, T.N., Lane, R.S. and Ribeiro, J.M.C. (2005) The transcriptome of the salivary glands of the female western black legged tick Ixodes pacificus (Acari: Ixodidae). Insect Biochem. Mol. Biol. 35, 1142-61.

• PMID: 16102420
• Manuscript = => Ix_pacificus_sialome.pdf [Note: This PDF files has linkouts to precomputed blast results.]
• Table1 = => table1.pdf
• Figure 1 = => Figure_1.pdf
• Figure 2 = => Figure_2.pdf
• Figure 3 = => Figure_3.pdf
• Figure 4 = => Figure_4.pdf
• Figure 5 = => Figure_5.pdf
• Figure 6 = => Figure_6.pdf
• Figure 7 = => Figure_7.pdf
• Figure 8 = => Figure_8.pdf
• Figure 9 = => Figure_9.pdf
• Figure 10 = => Figure_10.pdf
• Figure 11 = => Figure_11.pdf
• Fasta text file of all EST’s = => ip-fasta.txt

Review article with tick salivary gland protein annotations:

THE ROLE OF SALIVA IN TICK FEEDING (Ivo M.B Francischetti1, Anderson Sá-Nunes1, Ben J. Mans1, Isabel M. Santos2 and José M.C. Ribeiro1 (2009) Frontiers in Bioscience 14, 2051-2088).

• Full text
• Supplemental table

Salivary transcriptomes (sialome) of blood feeding insects:

• Aedes aegypti (Valenzuela, J. G., Pham, V. M., Garfield, M. K., Francischetti, I. M. and Ribeiro, J. M. C. (2002). Toward a description of the sialome of the adult female mosquito Aedes aegypti. Insect Biochem. Mol. Biol. 32, 1101-1122).
  • PMID: 12213246
  • Ae_aegypti_sialome.pdf
  • Ae_aegypti_sialome_Table1.ZIP [Correspond to publication data, not updated, Standalone]
  • Ae_aegypti_sialome_Table2.ZIP [Correspond to publication data, not updated, Standalone]
• Updated Aedes aegypti (Ribeiro, J.M.C., Arcà, B., Lombardo, F., Calvo, E., Phan, V.M., Chandra, P.K., Wikel, S.K. (2006). An annotated catalogue of salivary gland transcripts in the adult female mosquito, Aedes aegypti. BMC Genomics. 2007 8:6)
  • PMID: 17204158 (Open Access)
  • Supplemental Table S1
  • Supplemental Table S2
• Aedes albopictus (Arcà, B., Lombardo, F., Francischetti, I.M., Pham, V.M., Mestres-Simon, M., Andersen, J.F., Ribeiro, J.M. (2007) An insight into the sialome of the adult female mosquito Aedes albopictus. Insect Biochem Mol Biol. 37(2):107-27.)
  • PMID: 17244540
  • Supplemental Table S1
  • Supplemental Table S2
• Anopheles gambiae sialome (Francischetti, I. M., Valenzuela, J. G., Pham, V. M., Garfield, M. K. and Ribeiro, J. M. (2002). Toward a catalog for the transcripts and proteins (sialome) from the salivary gland of the malaria vector Anopheles gambiae. J. Exp. Biol. 205, 2429-51).
  • PMID: 12124367
  • An_gambiae_sialome.pdf
  • An_gambiae_sialome_TableI.ZIP [Correspond to publication data, not updated, Standalone]
  • An_gambiae_sialome_TableII.ZIP [Correspond to publication data, not updated, Standalone]
• Updated Anopheles gambiae sialome (Bruno Arcà, Fabrizio Lombardo, Jesus G. Valenzuela, Ivo M.B. Francischetti, Mario Coluzzi and Jose M.C. Ribeiro (2005) An updated catalog of salivary gland transcripts in the adult female mosquito, Anopheles gambiae (2005) J. Exp. Biol. 208, 3971-86.
  • PMID: 16215223
  • Manuscript
  • Fig 1 [Fig1.pdf]
  • Fig 2 [Fig2.pdf]
  • Fig 3 [Fig3.pdf]
  • Fig 4 [Fig4.pdf]
  • Fig 5 [Fig5.pdf]
  • Fig 6 [Fig6.pdf]
  • Fig 7 [Fig7.pdf]
  • Fig 8 [Fig8.pdf]
  • Table 1 [Table1.pdf]
  • Table 2 [Table2.pdf]
  • Table 3 [Table3.pdf]
  • Table 4 [Table4.xls]
  • Supplemental table [Sup-tableI.xls]
  • Fasta file of all EST’s (text file) [ag.fsa]
• Anopheles gambiae male sialotranscriptome (Calvo, E. Pham, V.M. Lombardo, F., Arcà and Ribeiro, J.M.C. (2006). The sialotranscriptome of adult male Anopheles gambiae mosquitoes. Insect Biochem Mol Biol. 36(7):570-5.
  • PMID: 16835022
  • Supplemental spreadsheet 1 [Sup-Table1.xls]
  • Supplemental spreadsheet 2 [Sup-Table2.xls]
  • Supplemental spreadsheet 3 [Sup-Table3.xls]
• Anopheles stephensi sialome (Valenzuela, J. G., Francischetti, I. M., Pham, V. M., Garfield, M. K. and Ribeiro, J. M. (2003). Exploring the salivary gland transcriptome and proteome of the Anopheles stephensi mosquito. Insect Biochem Mol Biol 33, 717-32).
  • PMID: 12826099
  • An_stephensi_sialome.pdf
  • An_stephensi_sialome_Table.ZIP [Correspond to publication data, not updated, Standalone]
• Anopheles funestus sialome (Calvo, E., Dao, A., Pham, V.M., and Ribeiro, J.M.C. (2007). An insight into the sialome of Anopheles funestus reveals an emerging pattern in anopheline salivary protein families. Insect Biochem Mol Biol 37, 164-75).
  • PMID: 17244545
  • Supplemental Table S1
  • Supplemental Table S2
• Anopheles darlingi (Calvo, E., Andersen, J., Francischetti, I. M., de, L. C. M., deBianchi, A. G., James, A. A., Ribeiro, J. M. and Marinotti, O. (2004). The transcriptome of adult female Anopheles darlingi salivary glands. Insect Mol Biol 13, 73-88).
  • PMID: 14728669
  • An_darlingi_sialome.pdf
  • An_darlingi_sialome_Table.zip [Correspond to publication data, not updated, Standalone]
• Culex pipiens quinquefasciatus (Ribeiro JM, Charlab R, Pham VM, Garfield M, Valenzuela JG. (2004). An insight into the salivary transcriptome and proteome of the adult female mosquito Culex pipiens quinquefasciatus. Insect Biochem Mol Biol. 2004 Jun;34(6):543-63.
  • PMID: 15147756
  • Cu_pipiens_quinquefasciatus_sialome.pdf
  • Cu_quinquefasciatus_sialome_Table.zip [Correspond to publication data, not updated, Standalone]
• Rhodnius prolixus (Ribeiro, J.M., Andersen, J., Silva-Neto, M.A., Pham, V.M., Garfield, M.K. and Valenzuela, J.G. (2004). Exploring the sialome of the blood-sucking bug Rhodnius prolixus. Insect Biochem Mol Biol 34, 61-79).
  • PMID: 14976983
  • Rh_prolixus_sialome.pdf
  • Rh_prolixus_sialome_Table.zip [Correspond to publication data, not updated, Standalone]
• Simulium vittatum (Andersen JF, Pham VM, Meng Z, Champagne DE, Ribeiro JM. (2009) Insight into the Sialome of the Black Fly, Simulium vittatum. J Proteome Res. Jan 23.)
  • PMID: 19166301
  • Supplemental Table S1
  • Supplemental Table S2
• Triatoma brasiliensis (Santos, A., Ribeiro, J.M., Lehane, M.J., Gontijo, N.F., Veloso, A.B., Sant'Anna, M.R., Nascimento Araujo R., Grisard, E.C., Pereira, M.H. (2007) The sialotranscriptome of the blood-sucking bug Triatoma brasiliensis (Hemiptera, Triatominae). Insect Biochem Mol Biol. 37:702-12).
  • PMID: 17550826 (Open Access)
  • Supplemental Table S1
  • Supplemental Table S2
• Triatoma infestans (Teresa C. F. Assumpção, Ivo M. B. Francischetti, John F. Andersen, Alexandra Schwarz, Jaime M. Santana and José M. C. Ribeiro (2008) An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas' disease. Insect Biochem Mol Biol. In press).
  • Supplemental Table S1
  • Supplemental Table S2 [Correspond to publication data, not updated, Standalone]
• Xenopsylla cheopis (Andersen, J.F., Hinnebusch, B.J., Lucas, D.A., Conrads, T.P., Veenstra, T.D., Pham, V.M., Ribeiro, J.M. (2007) An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots). BMC Genomics. 8:102.).
  • PMID: 17437641 (Open access)
  • Supplemental Table S1
  • Supplemental Table S2
  • Supplemental Table S3

Non-blood feeding sialotranscriptomes:

• Oncopeltus fasciatus, a seed feeding Hemiptera closely related to triatomines. (Francischetti, I.M., Lopes, A.H., Dias, F.A., Pham, V.M., Ribeiro, J.M. An insight into the sialotranscriptome of the seed-feeding bug, Oncopeltus fasciatus. Insect Biochem Mol Biol. 37(9):903-10.)
  • PMID: 17681229
  • Supplemental Table S1
  • Supplemental Table S2
    
• Toxorhynchites amboinensis a non-blood feeding mosquito. (E. Calvo, V. M. Pham and J. M. C. Ribeiro. An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito Insect Biochem Mol Biol. In press)
  • Supplemental Table S1
  • Supplemental Table S2

 

Whole Organism Transcriptome:

• Anopheles gambiae transcriptome analysis from whole blood-fed and non-blood fed adult female mosquitoes, as reported in Ribeiro, J. M. (2003). A catalogue of Anopheles gambiae transcripts significantly more or less expressed following a blood meal. Insect Biochem. Mol. Biol. 33, 865-82.
  • PMID: 12915178
  • An_gambiae_ESTome.pdf
  • An_gambiae_ESTome_Table.zip [Correspond to publication data, not updated, Standalone]

Poisonous animals:

• Transcriptome analysis of the tegument and spicule of the venomous caterpillar Lonomia obliqua (submitted).
  • Manuscript Figures: Figure1.pdf, Figure2.pdf, Figure3a.pdf, Figure3b.pdf, Figure4a.pdf, Figure4b.pdf, Figure5.pdf, Figure6.pdf, Figure7.pdf, Figure8.pdf, Figure9.pdf, Figure10.pdf, Figure11.pdf
  • Manuscript Tables: Table1.xls, Table2.xls, Table3.xls
  • DataFiles: Lo_obliqua_Table.zip [Correspond to publication data, not updated, Standalone]
• Transcriptome analysis of the venom gland of the snake Bitis gabonica (Francischetti, I. M., My-Pham, V., Harrison, J., Garfield, M. K. and Ribeiro, J. M. (2004). Bitis gabonica (Gaboon viper) snake venom gland: toward a catalog for the full-length transcripts (cDNA) and proteins. Gene 337, 55-69).
  • PMID: 15276202
  • Bi_gabonica_venonome.pdf
  • Bi_gabonica_venonome_Table.ZIP [Correspond to supplemental publication data, not updated, Standalone]
  • Bioinformatics supplement.pdf

---

ReAnoXcel: an Anopheles gambiae protein database Maintained - Updated Feb-22-2006

Improved Anopheles gambiae genome annotation through synthesis of ab initio and comparative gene prediction algorithms. Jun Li, Michelle M. Riehle, Yan Zhang, Jiannong Xu, Frederick Oduol, Shawn M. Gomez, Beatrix Ueberheide, Donald Hunt, Charles W. Roth, José M. C. Ribeiro, and Kenneth D. Vernick. S Genome Biol. 2006;7(3):R24

• PMID: 16569258
• The data set below refers to this new set of predicted genes, not ENSEMBL predictions.
• ReAnoXcel-Feb-2006.zip [27.7 MB] – download and unzip the files onto your computer. There are two tab delimited files (.tab) that can be imported to other databases or can be browsed by importing into Microsoft Excel.
  • ReAnoXcel-lookup.tab [58629778 bytes] - tab delimited lookup table
  • ReAnoXcel-Feb-2006.tab [134122881 bytes] - tab delimited ReAnoXcel data table - update of ReAnoXcel-Jan-2006.tab to reinclude duplicate genes by duplicating the record of the duplicate sequence (only changing the display id).
• Previous version: ReAnoXcel-Jan-2006-tab.zip [27.9 MB] is a hyperlink fix of ReAnoXcel-Nov2005-tab.zip [Removed].
  • ReAnoXcel-lookup.tab [55.9 MB] - tab delimited lookup table
  • ReAnoXcel-Jan-2006.tab [127 MB] - tab delimited ReAnoXcel data table - removed some duplicate genes (same sequence but located at different genome site, may be possible active transposable elements).
• The original data files are downloadable at: ftp://ftp.ncbi.nih.gov/pub/omes/ReAnoXcel/
  • Read the ReadMe.txt
• BLAST your sequence against the ReAnoXcel coding sequences
  

---

AnoXcel: an Anopheles gambiae protein database

• AnoXcel (Ribeiro, J. M., Topalis, P. and Louis, C. (2004). AnoXcel: an Anopheles gambiae protein database. Insect Mol Biol 13, 449-57).
• PMID: 15373803
• This data set refers to ENSEMBL annotated genes
• Get the last version of AnoXcel at Anobase