dCAS Download Instructions

dCAS is downloaded in a five step process in which the required components are installed followed by the dCAS automated cDNA sequence analysis application.

Required Components

NCBI BLAST executable files
BLAST databases (NR, GO, Mit-pla, RRNA, Conserved Domain Databases, and UniVec)
CAP3 sequence assembly program

Step 1 Install BLAST Executable Files Component	Download the platform-specific BLAST executable files to your desktop Unzip the file to a folder on your hard drive* When you run dCAS for the first time, you'll be asked to provide the location of the BLAST executable files *On a Windows machine, the download will extract to three folders: data, bin, doc. For simplicity, you may want to place these three folders in a new folder called 'BLAST'	NCBI
Step 2 Install BLAST Databases Component	Download the platform-specific BLAST database package to your desktop* Unzip the file to the same folder where you placed the BLAST executable files (created in Step 1) *Note: the BLAST database package is greater than 1.4 GB in size	PowerPC processor - Apple Macs Intel processor - Windows PC and Apple Macs
Step 3 Install CAP3 Sequence Assembly Component	Download the platform-specific CAP3 application from the CAP3 website to your desktop* Unzip the file to a new folder on your hard drive When you run dCAS for the first time, you'll be asked to provide the location of the CAP3 folder that you have created *As part of the download, you will be asked to agree with the CAP3 license terms from the owners of license, Iowa State University Research Foundation, Inc. and Michigan Technological University	CAP3
Step 4 Install Phred Application Component (Optional)	If you wish to use Phred for adding base-calling for DNA sequence trace files to dCAS, follow the installation instructions at the Phred website* *The download of Phred also involves compliance with a software user agreement. Phred software s emailed to users as described on the site	Phred
Step 5 Install dCAS Automated cDNA Sequence Analysis Application	Please select the target operating system and download the dCAS installer from the list on the right	Windows Mac OS X Linux
Step 6 Install dCAS Mate (Optional)	dCAS Mate is used with dCAS for parallel processing. It should be installed on the slave computer, which will be controlled by dCAS through local network. Please select the target operating system and download the dCAS Mate installer from the list on the right	Windows Mac OS X Linux
Additional Materials:	We provide sample sequences for testing your copy of dCAS. Download the zipped folder of sample sequence files by following the link at right Unzip the files to a location on your hard drive	Sample Sequences